Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Ouchi, Kazuki
Hosha Kagaku, (49), p.3 - 7, 2024/03
I introduce the elucidation of the deposition following the oxidation state of uranium and the electrochemical behavior of uranium(IV) chloride in an ionic liquid-organic mixture, as a basic study of in-solution reactions. In addition, I introduce the development of separation methods for actinides using a microchemical chip and polyacrylamide gel electrophoresis, as an applied study for quantitative analytical methods for small amounts of samples.
raysYokota, Yuichiro; Shikazono, Naoya; Tanaka, Atsushi; Hase, Yoshihiro; Funayama, Tomoo; Wada, Seiichi; Inoue, Masayoshi*
Radiation Research, 163(5), p.520 - 525, 2005/05
Times Cited Count：20 Percentile：50.54(Biology)Higher plants are generally more tolerant to ionizing radiation than mammals. To explore the radiation tolerance of higher plants, the amount of DNA double-strand breaks (DSBs) induced by -rays was investigated in tobacco BY-2 cells and compared with that investigated in Chinese hamster ovary (CHO)-K1 cells as a reference. The resulting DNA fragments were separated by pulsed-field gel electrophoresis and stained with SYBR Green I. Initial DSB yield was then quantified from the fraction of DNA fragments shorter than 1.6 Mbp based on the assumption of random distribution of DSBs. The DSB yield in tobacco BY-2 cells (2.0 
0.1 DSBs Gbp
Gy) was only one-third of that in CHO-K1 cells. Furthermore, the calculated number of DSBs per diploid cell irradiated with -rays of mean lethal dose was five times greater in tobacco BY-2 cells (263.2 13.2) than in CHO-K1 cells. These results suggest that the radiation tolerance of tobacco BY-2 cells appears to be due to not only a lower induction of DNA damage but also a more efficient repair of the induced DNA damage.
by pulsed-field gel electrophoresisKikuchi, Masahiro; Narumi, Issei; Kobayashi, Yasuhiko
JAERI-Conf 2002-005, P. 185, 2002/03
The most striking feature of the radioresistant bacterium 
is that it can mend over 100 double-strand breaks of genomic DNA during post-irradiation incubation. This process can be clearly visualized using pulsed-field gel electrophoresis (PFGE). By a combination of protein synthesis inhibition treatment and PFGE analysis, it was possible to estimate an initial period required for induction of DNA repair proteins (induction time) and a total period required for completing DNA repair (repair time). PFGE is a powerful tool to analyze DNA damage and its repair process.
Haraga, Tomoko; Marumo, Kazuki*; Saito, Takumi*; Shibukawa, Masami*; Saito, Shingo*
no journal, ,
no abstracts in English
Ouchi, Kazuki
no journal, ,
I describe on the elucidation of the deposition following the oxidation state of uranium and the electrochemical behavior of uranium(IV) chloride in an ionic liquid-organic mixture, as a basic study of in-solution reactions. In addition, I describe on the development of separation methods for actinides using a microchemical chip and polyacrylamide gel electrophoresis, as an applied study for quantitative analytical methods for small amounts of samples.
